NuCLEANase®
High-performance enzyme for nucleic acid removal in industrial processes

NuCLEANase®
Enzymatic removal of DNA, for regulatory compliance and process efficiency improvements of fermentative processes
NuCLEANase® is an endonuclease, which can be used for the enzymatic removal of nucleic acids in a large variety of bioprocesses. A frequent application is the reduction of residual NuCLEANase® DNA for regulatory compliance of fermentation processes.
NuCLEANase® can contribute to a more efficient production process of microbial derived products. It reduces the nucleic acid induced viscosity, which makes the recovery process easier.
As NuCLEANase® is beneficial for a wide range of biotech applications it comes as a food- and tech-grade formulation. The food-grade is halal/kosher certified and listed on the FDAs GRAS Inventory List.

Degrades DNA and RNA molecules into small fragments (2-5 base pairs)
Highly active over a wide range of operating conditions
Positive GRAS Evaluation from US FDA

After submitting a GRAS notification for NuCLEANase® (GRN No.1146) to the US FDA, c-LEcta has received a "No questions letter” from the authority, which is publicly available within the GRAS Inventory List. The GRAS Notification designates that the usage of NuCLEANase® in food applications is considered safe under the conditions of its intended use.
For which Application Field do you need NuCLEANase®?

In fermentative derived products, NuCLEANase® can be used for the removal of residual DNA from genetically modified production hosts for regulatory compliance.

In phage manufacturing processes NuCLEANase® is used to specifically remove nucleic acids from cell lysates to reduce the viscosity and aggregate formation and hence facilitates the purification of the phages.

In fermentation processes, NuCLEANase® can be used to reduce the nucleic acid induced viscosity in fermentation broths, enabling a significantly more efficient process.
Order & Test NuCLEANase® Now!
- Food grade
High-Performance Endonuclease for Industrial Applications
Special sample sizes for further testing and small piloting. For bulk amounts please inquire the prices and contact us.Activity: ≥ 30 MU/L¹
Reaction conditions: optimum at 37 °C , pH 8.0 – 9.0
Cofactor: 1 - 2 mM Mg2+ cations for optimal activity
Formulation: potassium phosphate buffer pH 8.0, 50 % (v/v) glycerol
Storage temperature: -20 °C¹Unit definition: One unit (U) digests salmon sperm DNA to acid-soluble oligonucleotides equivalent to ΔA 260 nm of 1.0 in 30 min at pH 8.0 at 37 °C.- Manufacturing free of animal derived raw materials and antibiotics
- Net price excluding VAT
Choose your size for NuCLEANase® Food grade:
- Tech grade
High-Performance Endonuclease for Industrial Applications
Special sample sizes for further testing and small piloting. For bulk amounts please inquire the prices and contact us.Activity: ≥ 30 MU/L¹
Reaction conditions: optimum at 37 °C , pH 8.0 – 9.0
Cofactor: 1 - 2 mM Mg2+ cations for optimal activity
Formulation: potassium phosphate buffer pH 8.0, 50 % (v/v) glycerol
Storage temperature: -20 °C¹Unit definition: One unit (U) digests salmon sperm DNA to acid-soluble oligonucleotides equivalent to ΔA 260 nm of 1.0 in 30 min at pH 8.0 at 37 °C.- Manufacturing free of animal derived raw materials and antibiotics
- Net price excluding VAT
Choose your size for NuCLEANase® Tech grade:
- Free sample
High-Performance Endonuclease for Industrial Applications
Reaction conditions: optimum at 37 °C , pH 8.0 – 9.0
Cofactor: 1 - 2 mM Mg2+ cations for optimal activity
Formulation: potassium phosphate buffer pH 8.0, 50 % (v/v) glycerol
Storage temperature: -20 °C
- Manufacturing free of animal derived raw materials and antibiotics
- Net price excluding VAT

High-Performance Endonuclease for Industrial Applications
Reaction conditions: optimum at 37 °C , pH 8.0 – 9.0
Cofactor: 1 - 2 mM Mg2+ cations for optimal activity
Formulation: potassium phosphate buffer pH 8.0, 50 % (v/v) glycerol
Storage temperature: -20 °C
- Manufacturing free of animal derived raw materials and antibiotics
- Net price excluding VAT

Get in touch with our NuCLEANase® expert team!
We are looking forward to support you with your question. It would our pleasure to discuss NuCLEANase® and how it can be used for your production processes.
Need support? Contact me!

Simon Ulm
NuCLEANase® Sales