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NuCLEANase® for Phage Production

In phage manufacturing processes NuCLEANase® is used to specifically remove nucleic acids from cell lysates to  reduces the viscosity and  aggregate formation  and hence facilitates the purification of the phages.

Bacterial viruses known as phages are among the most common and diverse entities in the biosphere. Phages are harmless to humans, animals, and plants and only infect bacterial cells selectively. Already today phages are used as alternative for antibiotics and chemical substances in US FDA approved products.

The production of phages makes use of their natural life cycle, in which the phages first inject their genetic material into the host and then hijack the host's cellular machinery to create new viral particles. After enough progeny has been produced, the phages will burst out of the host cell, causing it to lyse.

Challenge
The cell lysis step in the production process of bacteriophages inevitably leads to a complex mixture of cell debris, phages, and large quantities of nucleic acids. The strongly negatively charged DNA and RNA  molecules can easily build aggregates with bacteriophages which may lead to significant product losses in commonly applied recovery steps such as precipitation, centrifugation, and filtration. The recovery is further challenged by the viscosity increase caused by the release of nucleic acids. 

Phages

Benefits

Decreases viscosity

Reduces aggregation of phages

Enables a more efficient and simpler processing

Solution
NuCLEANase®, makes phage production simpler and more efficient. By digesting all forms of nucleic acids, shortly after the lysis step, more phages will stay in solution. In addition, the digestion of nucleic acids will help to reduce the viscosity of the lysate. Both aspects will enable a significantly more efficient primary recovery and overall purification of the bacteriophages.

Order & Test NuCLEANase® Now!

  • Food grade

    High-Performance Endonuclease for Industrial Applications

    Special sample sizes for further testing and small piloting. For bulk amounts please inquire the prices and contact us. 

    Activity: ≥ 30 MU/L¹
    Reaction conditions: optimum at 37 °C , pH 8.0 – 9.0
    Cofactor: 1 - 2 mM Mg2+ cations for optimal activity
    Formulation: potassium phosphate buffer pH 8.0, 50 % (v/v) glycerol
    Storage temperature: -20 °C
     
    ¹Unit definition: One unit (U) digests salmon sperm DNA to acid-soluble oligonucleotides equivalent to ΔA 260 nm of 1.0 in 30 min at pH 8.0 at 37 °C.
     
    • Manufacturing free of animal derived raw materials and antibiotics
    • Net price excluding VAT
    NuCLEANase_food-grade_all-1

    Choose your size for NuCLEANase® Food grade:

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  • Tech grade

    High-Performance Endonuclease for Industrial Applications

    Special sample sizes for further testing and small piloting. For bulk amounts please inquire the prices and contact us. 

    Activity: ≥ 30 MU/L¹
    Reaction conditions: optimum at 37 °C , pH 8.0 – 9.0
    Cofactor: 1 - 2 mM Mg2+ cations for optimal activity
    Formulation: potassium phosphate buffer pH 8.0, 50 % (v/v) glycerol
    Storage temperature: -20 °C
     
    ¹Unit definition: One unit (U) digests salmon sperm DNA to acid-soluble oligonucleotides equivalent to ΔA 260 nm of 1.0 in 30 min at pH 8.0 at 37 °C.
     
    • Manufacturing free of animal derived raw materials and antibiotics
    • Net price excluding VAT
    NuCLEANase_tech-grade_all

    Choose your size for NuCLEANase® Tech grade:

    Back to the section
  • Free sample

High-Performance Endonuclease for Industrial Applications

Special sample sizes for further testing and small piloting. For bulk amounts please inquire the prices and contact us. 

Activity: ≥ 30 MU/L¹
Reaction conditions: optimum at 37 °C , pH 8.0 – 9.0
Cofactor: 1 - 2 mM Mg2+ cations for optimal activity
Formulation: potassium phosphate buffer pH 8.0, 50 % (v/v) glycerol
Storage temperature: -20 °C
 
¹Unit definition: One unit (U) digests salmon sperm DNA to acid-soluble oligonucleotides equivalent to ΔA 260 nm of 1.0 in 30 min at pH 8.0 at 37 °C.
 
  • Manufacturing free of animal derived raw materials and antibiotics
  • Net price excluding VAT
NuCLEANase_food-grade_all-1

Choose your size for NuCLEANase® Food grade:

High-Performance Endonuclease for Industrial Applications

Special sample sizes for further testing and small piloting. For bulk amounts please inquire the prices and contact us. 

Activity: ≥ 30 MU/L¹
Reaction conditions: optimum at 37 °C , pH 8.0 – 9.0
Cofactor: 1 - 2 mM Mg2+ cations for optimal activity
Formulation: potassium phosphate buffer pH 8.0, 50 % (v/v) glycerol
Storage temperature: -20 °C
 
¹Unit definition: One unit (U) digests salmon sperm DNA to acid-soluble oligonucleotides equivalent to ΔA 260 nm of 1.0 in 30 min at pH 8.0 at 37 °C.
 
  • Manufacturing free of animal derived raw materials and antibiotics
  • Net price excluding VAT
NuCLEANase_tech-grade_all

Choose your size for NuCLEANase® Tech grade:

Get in touch with our NuCLEANase® 
expert team!

We are looking forward to support you with your question. It would our pleasure to discuss NuCLEANase® and how it can be used for your production processes.

Need support? Contact me!

Simon Ulm

Simon Ulm
NuCLEANase® Sales