What is NuCLEANase®?

NuCLEANase® is a genetically engineered endonuclease from Serratia marcescens. It catalyzes the hydrolysis of phosphodiester bonds between nucleotides, leaving short fragments with a length of 2 to 5 bases and a 5’-monophosphate end. NuCLEANase® cleaves all forms of DNA/RNA (single-stranded, double-stranded, linear, and circular).

In the food or feed sector, for example, the applications are manifold. NuCLEANase® enables the efficient removal of DNA in food and feed enzymes as well as other fermentation products, thus ensuring their compliance with the relevant regulatory guidelines. Likewise, NuCLEANase® can boost animal growth by increasing the bioavailability of nucleotides in animal feed.

As a leading supplier of enzyme technologies, c-LEcta is offering a nuclease for the first time in industrial quantities at affordable prices. Depending on the application and the end product, NuCLEANase® is available in tech-grade or food-grade quality; with the latter being halal/kosher-certified. The production host is a QPS strain and no antibiotics or animal-derived materials are used in the manufacturing process.


Activity: ≥ 30 MU/L¹

Reaction conditions: optimum at 37 °C, pH 8.0 – 9.0

Cofactor: 1 - 2 mM Mg²+ cations for optimal activity

Formulation: potassium phosphate buffer pH 8.0, 50 % (v/v) glycerol

Storage temperature: -20 °C

¹Unit definition: One unit (U) digests salmon sperm DNA to acid-soluble oligonucleotides equivalent to ΔA 260 nm of 1.0 in 30 min at pH 8.0 at 37 °C.

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    Simon Ulm
    Simon UlmNuCLEANase Sales+49 341 355 214 377

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