NuCLEANase® - Enzyme Characteristics
Activity: ≥ 30 MU/L¹
Molecular weight (calculated): 27 kDa
Reaction conditions: optimum at 37 °C, pH 8.0 – 9.0
Cofactor: 1 - 2 mM Mg²+ cations for optimal activity (not included in the enzyme formulation)
Formulation: potassium phosphate buffer pH 8.0, 50 % (v/v) glycerol
Storage temperature: -20 °C
¹Unit definition: One unit (U) digests salmon sperm DNA to acid-soluble oligonucleotides equivalent to ΔA 260 nm of 1.0 in 30 min at pH 8.0 at 37 °C.
For more detailed information on the enzyme parameters and for more guidance on the implementation in fermentation processes please request the Application note.
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