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NuCLEANase® for Viscosity Reduction of Fermentation Broths

In fermentation processes, NuCLEANase® can be used to reduce the nucleic acid induced viscosity in fermentation broths, enabling a significantly more efficient product recovery.

The strong tendency of DNA molecules to increase viscosity arises from their molecular characteristics. The great length of DNA strands causes them to entangle with one another, leading to increased viscosity. In addition, the negatively charged phosphate backbone is surrounded by a layer of less dynamic water molecules, further contributing to the viscosity.

Challenge
High viscosity of fermentation broths greatly impacts the efficiency the recovery process. The Separation of solids from the broth by centrifugation or filtration becomes less effective due to the increased resistance of the fluid, requiring more energy for pumping. Also, a faster fouling of membranes is often seen with higher viscosities.

Solution
NuCLEANase® is a simple and efficient solution for the reduction of nucleic acid induced viscosity. The addition of 10 kU/ml of NuCLEANase® to the culture broth of a large-scale microbial Bacillus sp. Fermentation reduced the viscosity of the broth by 88% within one hour after addition of the enzyme. As a result of the lower viscosity the transmembrane pressure during the following micro-filtration step was lower over a longer time and easier to control. This resulted in a 22 % higher overall throughput and a reduction of the filtration time by 7 hours (25 %).

c-LEcta_Fermentation Process

Benefits

Reduces viscosity by cleaving lengthy DNA molecules into small nucleotides of 2-5 base pairs

Cost-effective add-on to existing processes

Improves DSP and overall process efficiency

Fermentation Processes

High concentrations of nucleic acids are often the reason for a viscosity increase of fermentation broths. Consequently, subsequent recovery steps become more challenging and less efficient.

Check out this case study and see how NuCLEANase® reduced the viscosity and significantly improved the efficiency of the primary recovery step of a large scale Bacilus application.

Order & Test NuCLEANase® Now!

  • Food grade

    High-Performance Endonuclease for Industrial Applications

    Special sample sizes for further testing and small piloting. For bulk amounts please inquire the prices and contact us. 

    Activity: ≥ 30 MU/L¹
    Reaction conditions: optimum at 37 °C , pH 8.0 – 9.0
    Cofactor: 1 - 2 mM Mg2+ cations for optimal activity
    Formulation: potassium phosphate buffer pH 8.0, 50 % (v/v) glycerol
    Storage temperature: -20 °C
     
    ¹Unit definition: One unit (U) digests salmon sperm DNA to acid-soluble oligonucleotides equivalent to ΔA 260 nm of 1.0 in 30 min at pH 8.0 at 37 °C.
     
    • Manufacturing free of animal derived raw materials and antibiotics
    • Net price excluding VAT
    NuCLEANase_food-grade_all-1

    Choose your size for NuCLEANase® Food grade:

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  • Tech grade

    High-Performance Endonuclease for Industrial Applications

    Special sample sizes for further testing and small piloting. For bulk amounts please inquire the prices and contact us. 

    Activity: ≥ 30 MU/L¹
    Reaction conditions: optimum at 37 °C , pH 8.0 – 9.0
    Cofactor: 1 - 2 mM Mg2+ cations for optimal activity
    Formulation: potassium phosphate buffer pH 8.0, 50 % (v/v) glycerol
    Storage temperature: -20 °C
     
    ¹Unit definition: One unit (U) digests salmon sperm DNA to acid-soluble oligonucleotides equivalent to ΔA 260 nm of 1.0 in 30 min at pH 8.0 at 37 °C.
     
    • Manufacturing free of animal derived raw materials and antibiotics
    • Net price excluding VAT
    NuCLEANase_tech-grade_all

    Choose your size for NuCLEANase® Tech grade:

    Back to the section
  • Free sample

High-Performance Endonuclease for Industrial Applications

Special sample sizes for further testing and small piloting. For bulk amounts please inquire the prices and contact us. 

Activity: ≥ 30 MU/L¹
Reaction conditions: optimum at 37 °C , pH 8.0 – 9.0
Cofactor: 1 - 2 mM Mg2+ cations for optimal activity
Formulation: potassium phosphate buffer pH 8.0, 50 % (v/v) glycerol
Storage temperature: -20 °C
 
¹Unit definition: One unit (U) digests salmon sperm DNA to acid-soluble oligonucleotides equivalent to ΔA 260 nm of 1.0 in 30 min at pH 8.0 at 37 °C.
 
  • Manufacturing free of animal derived raw materials and antibiotics
  • Net price excluding VAT
NuCLEANase_food-grade_all-1

Choose your size for NuCLEANase® Food grade:

High-Performance Endonuclease for Industrial Applications

Special sample sizes for further testing and small piloting. For bulk amounts please inquire the prices and contact us. 

Activity: ≥ 30 MU/L¹
Reaction conditions: optimum at 37 °C , pH 8.0 – 9.0
Cofactor: 1 - 2 mM Mg2+ cations for optimal activity
Formulation: potassium phosphate buffer pH 8.0, 50 % (v/v) glycerol
Storage temperature: -20 °C
 
¹Unit definition: One unit (U) digests salmon sperm DNA to acid-soluble oligonucleotides equivalent to ΔA 260 nm of 1.0 in 30 min at pH 8.0 at 37 °C.
 
  • Manufacturing free of animal derived raw materials and antibiotics
  • Net price excluding VAT
NuCLEANase_tech-grade_all

Choose your size for NuCLEANase® Tech grade:

Get in touch with our NuCLEANase® 
expert team!

We are looking forward to support you with your question. It would our pleasure to discuss NuCLEANase® and how it can be used for your production processes.

Need support? Contact me!

Simon Ulm

Simon Ulm
NuCLEANase® Sales